Primer

Part:BBa_K4496007:Design

Designed by: Larissa de Souza Kawanisi, Awana da Silva Lima, Paula Macedo Cunha, Gabriel Kimijima Itaziki   Group: iGEM22_USP-EEL-Brazil   (2022-09-20)


Jararagin gene amplification primers for pPIC9K (REV)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This primer was designed from the jararhagin sequence optimized for Pichia Pastoris and the plasmid pPICK9k. For this, a 5 amino acid REV primer tail was designed, using the pPICK9k plasmid map, and joined it with the last 5 amino acids (in reverse primer mode) of the jararhagin gene optimized for Pichia pastoris. This sequence was tested for hairpin.


Source

https://parts.igem.org/Part:BBa_K4496000

References